IHC of p21 on an FFPE Colon Carcinoma Tissue

IHC of p21 on an FFPE Colon Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation p21 is a potent cyclin-dependent kinase inhibitor. The p21 protein binds to and inhibits the activity of cyclin-CDK2 or -CDK1 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a varity of stimuli. In addition to growth arrest, p21 can mediate cellular senescence. p21 can also interact with proliferating cell nuclear antigen (PCNA) and
plays a regulatory role in S phase DNA replication and DNA damage repair.
Normal cells typically display a rather intense nuclear p21 expression. Loss of p21 expression has been associated with poor prognosis in several carcinomas including Gastric Carcinoma, Non-Small Cell Lung Carcinoma and Thyroid Carcinoma.
Antibody Type Mouse Monoclonal Clone DCS-60.2
Isotype IgG2a Reactivity Paraffin, Frozen
Localization Cytoplasmic, Nuclear Control Tonsil, Colon, Fallopian Tube, Breast Cancer, Colon Carcinoma
Presentation p21 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2063 Prediluted Ready-To-Use 3.0 ml
BSB 2064 Prediluted Ready-To-Use 7.0 ml
BSB 2065 Prediluted Ready-To-Use 15.0 ml
BSB 2066 Concentrated 1:50-1:200 0.1 ml
BSB 2067 Concentrated 1:50-1:200 0.5 ml
BSB 2068 Concentrated 1:50-1:200 1.0 ml
BSB 2069 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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