IHC of NGFR on an FFPE Breast Fibroadenoma Tissue

IHC of NGFR on an FFPE Breast Fibroadenoma Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationNGFR (Nerve Growth Factor Receptor), also termed p75 or CD271, is the low-affinity NGFR (LNGFR) which binds NGF and other neurotrophins, including BDNF, NT3 and NT4/5 with similar low-affinity. NGFR p75 is a 75 kD transmembrane glycoprotein that is mainly expressed in Schwann cells and neurons and in a variety of non-neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE-like proteases and the JNK pathway. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells). NGFR is expressed not only in sympathetic and sensory neurons, but also in various neural crest cell or tumor derivatives such as melanocytes, Melanomas, Neuroblastomas, Pheochromocytomas, Neurofibromas, and neurotized nevi (Type C melanocytes). It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system. Studies in Prostate and Urothelial Cancer suggest that NGFR may act as a tumor suppressor, negatively regulating cell growth and proliferation. NGFR labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts and, thus, aids in the diagnosis of malignancy in the breast.
Antibody TypeMouse MonoclonalCloneBSB-18
IsotypeIgG1ReactivityParaffin, Frozen
LocalizationCytoplasmicControlNeuroblastoma, Brain, Breast, Prostate, CNS Tumor
PresentationNGFR is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6289PredilutedReady-To-Use3.0 ml
BSB 6290PredilutedReady-To-Use7.0 ml
BSB 6291PredilutedReady-To-Use15.0 ml
BSB 6292Concentrated1:250-1:10000.1 ml
BSB 6293Concentrated1:250-1:10000.5 ml
BSB 6294Concentrated1:250-1:10001.0 ml
BSB 6295Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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