IHC of Neutrophil Elastase on an FFPE Spleen Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

Neutrophil elastase (NE) is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. In humans, neutrophil elastase is encoded by the ELANE gene, which resides on chromosome 19. Secreted by neutrophils and macrophages during inflammation, it destroys bacteria and host tissue. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin.  Neutrophil elastase may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. Neutrophil elastase is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Mutations of the ELANE gene cause severe congenital neutropenia, which is a failure of neutrophils to mature.

Neutrophil elastase  is expressed in developing granulocytes in the bone marrow, stored in the azurophilic  granules of mature neutrophils and released upon neutrophil activation or disintegration. Despite the fact that neutrophils carry large amount of NE, and are capable of synthesizing a variety of proteins, the NE gene is not expressed in mature neutrophils. Also, NE can be used as an additional marker to differentiate the involvement of neutrophilic myeloid cells. This marker is useful for differentiating leukemic infiltrates of myeloproliferative processes in lymph nodes and other organs from undifferentiated carcinomas and/or histiocytic sarcomas or large cell lymphomas. Neutrophil elastase is a useful aid for classification of acute myeloid leukemia and extramedullary myeloid cell tumors.

Antibody Type Rabbit Monoclonal Clone EP223
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Bone Marrow, Spleen, Liver, Granulocytic Leukemia
Presentation Neutrophil Elastase is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2999 Prediluted Ready-To-Use 3.0 ml
BSB 3100 Prediluted Ready-To-Use 7.0 ml
BSB 3101 Prediluted Ready-To-Use 15.0 ml
BSB 3102 Concentrated 1:50-1:200 0.1 ml
BSB 3103 Concentrated 1:50-1:200 0.5 ml
BSB 3104 Concentrated 1:50-1:200 1.0 ml
BSB 3105 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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