IHC of MUC1 on an FFPE Breast Carcinoma Tissue

IHC of MUC1 on an FFPE Breast Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Mucin 1, also known as MUC1, is a member of the mucin family and encodes a membrane-bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, the degree of glycosylation varying with cell type. Mucins are high molecular-weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical aggressions. The MUC1 protein serves a protective function by binding to pathogens and also functions in a cell-signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively-spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
MUC1 is a large cell, surface-mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed in lactating mammary glands and overexpressed in more than 90% Breast Carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.
Antibody Type Rabbit Monoclonal Clone EP85
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Breast, Colon, Kidney, Fallopian Tube, Tonsil, Colon Adenocarcinoma
Presentation MUC1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6939 Prediluted Ready-To-Use 3.0 ml
BSB 6940 Prediluted Ready-To-Use 7.0 ml
BSB 6941 Prediluted Ready-To-Use 15.0 ml
BSB 6942 Concentrated 1:50-1:200 0.1 ml
BSB 6943 Concentrated 1:50-1:200 0.5 ml
BSB 6944 Concentrated 1:50-1:200 1.0 ml
BSB 6945 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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