IHC of MUC1 on an FFPE Breast Carcinoma Tissue

IHC of MUC1 on an FFPE Breast Carcinoma Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationMucin 1, also known as MUC1, is a member of the mucin family and encodes a membrane-bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, the degree of glycosylation varying with cell type. Mucins are high molecular-weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium from chemical and mechanical aggressions. The MUC1 protein serves a protective function by binding to pathogens and also functions in a cell-signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively-spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
MUC1 is a large cell, surface-mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed in lactating mammary glands and overexpressed in more than 90% Breast Carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.
Antibody TypeRabbit MonoclonalCloneEP85
IsotypeIgGReactivityParaffin, Frozen
LocalizationCytoplasmicControlBreast, Colon, Kidney, Fallopian Tube, Tonsil, Colon Adenocarcinoma
PresentationMUC1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 6939PredilutedReady-To-Use3.0 ml
BSB 6940PredilutedReady-To-Use7.0 ml
BSB 6941PredilutedReady-To-Use15.0 ml
BSB 6942Concentrated1:50-1:2000.1 ml
BSB 6943Concentrated1:50-1:2000.5 ml
BSB 6944Concentrated1:50-1:2001.0 ml
BSB 6945Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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