|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||The Mouse/Rabbit ImmunoDetector Alkaline Phosphatase/ ALK Magenta Detection System is a Biotin-Streptavidin-Alkaline Phosphatase Detection System that allows for the demonstration of antigens in paraffin-embedded tissue, cryostat sections, cytosmears, and cell preparations. The increased sensitivity of ImmunoDetector AP/ALK Magenta Detection System allows for rapid staining procedures without compromises in the quality of stains.
The ImmunoDetector AP Blocker is used to block the endogenous Alkaline Phosphatase enzymes that naturally occur in cells and tissue sections without affecting antigens or nucleic acids. The Mouse/Rabbit ImmunoDetector Alkaline Phosphatase Detection System is suitable for use with mouse (IgG and IgM) and rabbit primary monoclonal and polyclonal antibodies. The substrate chromogen is the final step in the detection portion; it enables the antibody-antigen-enzyme complex to be viewed under the light microscope. This occurs when Alk Magenta, in the presence of Alkaline Phosphatase, gets deposited at the site of the target antigen or nucleic acid, producing a magenta color that is partially soluble in organic solvents, and therefore care should be taken when mounting with permanent mounting media (please refer to the recommended permanent mounting protocol on reverse). The ImmunoDetector AP/ALK Magenta Detection System kits are universal kits and therefore work equally well with prediluted and concentrated antibodies from different vendors.
|Presentation||ImmunoDetector Alkaline Phosphatase Detection System contains an Alkaline Phosphatase Blocker solution, a Biotinylated Anti-Mouse and Anti-Rabbit immunoglobulins solution, a Streptavidin conjugated to Alkaline Phosphatase solution, an ALK Magenta chromogen solution, an ALK Magenta Activator solution, and an ALK Magenta Buffer-Substrate solution. All the components are buffered with stabilizers and a non-azide anti-microbial.|