Ancillaries

For IVD Use
For Research Use Only
Safety Data Sheet

SDS

Intended Use For Mohs In Vitro Diagnostic Use
Summary and Explanation The Mohs ImmunoDigestor is used for the enzymatic digestion of frozen tissues prior to immunohistochemical (IHC) [procedures.

The enzymatic digestion of frozen tissues fixed with acetone or NBF 10%, improves accessibility of antibodies to epitopes within tissue sections.  In the case of IHC, proteolytic digestion exposes certain epitopes which have been masked during fixation, especially with NBF10%.

Presentation The Mohs ImmunoDigestor is provided in liquid form ready-to-use.  It contains optimized Proteinase K, a buffer solution, detergents, and sodium azide anti-microbial.
Availability
CAT.# DILUTION VOL
BSB 0324 Ready-To-Use 15.0 ml
BSB 0325 Ready-To-Use 50.0 ml
BSB 0326 Ready-To-Use 100.0 ml

Recommended Protocol

  1. For Frozen Mohs sections fixed for 2 min. with Acetone or 10% NBF 10%, incubate slides with ImmunoDNA Digestor for 1 minute at 37°C or at room temperature.
  2. Rinse slides briefly in distilled or deionized water (10-20 seconds).
  3. Proceed with Mohs IHC protocol.

Abbreviated Mohs Immunohistochemical Protocol

Step Mohs PolyDetector HRP Green or DAB 5 min Protocol Mohs PolyDetector HRP Green or DAB 10 min Protocol
Peroxidase/AP Blocker 0.5 min. 0.5 min.
Primary Antibody 2 min 4 min.
1st Step Detection 1 min 3 min.
Substrate-Chromogen 1 min 2 min.
Counterstain / Coverslip 0.5 min 0.5 min.
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