|Intended Use||For Mohs In Vitro Diagnostic Use|
|Summary and Explanation||The Mohs ImmunoDigestor is used for the enzymatic digestion of frozen tissues prior to immunohistochemical (IHC) [procedures.
The enzymatic digestion of frozen tissues fixed with acetone or NBF 10%, improves accessibility of antibodies to epitopes within tissue sections. In the case of IHC, proteolytic digestion exposes certain epitopes which have been masked during fixation, especially with NBF10%.
|Presentation||The Mohs ImmunoDigestor is provided in liquid form ready-to-use. It contains optimized Proteinase K, a buffer solution, detergents, and sodium azide anti-microbial.|
- For Frozen Mohs sections fixed for 2 min. with Acetone or 10% NBF 10%, incubate slides with ImmunoDNA Digestor for 1 minute at 37°C or at room temperature.
- Rinse slides briefly in distilled or deionized water (10-20 seconds).
- Proceed with Mohs IHC protocol.
Abbreviated Mohs Immunohistochemical Protocol
|Step||Mohs PolyDetector HRP Green or DAB 5 min Protocol||Mohs PolyDetector HRP Green or DAB 10 min Protocol|
|Peroxidase/AP Blocker||0.5 min.||0.5 min.|
|Primary Antibody||2 min||4 min.|
|1st Step Detection||1 min||3 min.|
|Substrate-Chromogen||1 min||2 min.|
|Counterstain / Coverslip||0.5 min||0.5 min.|