IHC of MLH1 on an FFPE Colon Carcinoma Tissue

IHC of MLH1 on an FFPE Colon Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation MLH1 is a mismatch repair gene of around 87 kDa, commonly associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC). This gene was identified as a locus frequently mutated in HNPCC. It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. In a high proportion of patients with microsatellite instability (MSI-H), the MLH1 protein is typically deficient. This protein deficiency is linked to the autosomal dominant condition of Hereditary Non-Polyposis Colon Cancer. The anti-MLH1antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite-unstable have a better prognosis than their microsatellite stable counterparts.
Antibody Type Mouse Monoclonal Clone G168-728
Isotype IgG2a/K Reactivity Paraffin, Frozen
Localization Nuclear Control Testis, Tonsil, Colon, Kidney, Colon Carcinoma
Presentation Anti-MLH1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 5764 Prediluted Ready-To-Use 3.0 ml
BSB 5765 Prediluted Ready-To-Use 7.0 ml
BSB 5766 Prediluted Ready-To-Use 15.0 ml
BSB 5767 Concentrated 1:10-1:50 0.1 ml
BSB 5768 Concentrated 1:10-1:50 0.5 ml
BSB 5769 Concentrated 1:10-1:50 1.0 ml
BSB 5770 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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