IHC of MiTF on an FFPE Melanoma Tissue

IHC of MiTF on an FFPE Melanoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Microphthalmia-associated Transcription Factor (MiTF) is a basic helix-loop-helix leucine zipper transcription factor involved in melanocyte and osteoclast development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg Syndrome Type II, Type IIa and Tietz Syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 Melonoma cells (but not other Melanoma cell lines), as well as mast cells and heart cells. MiTF plays a critical role in the differentiation of various cell types such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. This antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia. It is useful in identifying Malignant Melanoma, and distinguishing mast cell lesions from lesions of myeloid derivation. A relatively rare class of tumors known as PEComas (tumors showing perivascular epithelioid cell differentiation) express MiTF in a high percentage of cases (~90%).
Antibody Type Mouse Monoclonal Clone C5/D5
Isotype IgG1/K Reactivity Paraffin, Frozen
Localization Nuclear Control Skin, Melanoma
Presentation Anti-MiTF is cocktail of two mouse monoclonal antibodies derived from cell culture supernatant that are concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6247 Prediluted Ready-To-Use 3.0 ml
BSB 6248 Prediluted Ready-To-Use 7.0 ml
BSB 6249 Prediluted Ready-To-Use 15.0 ml
BSB 6250 Concentrated 1:250-1:1000 0.1 ml
BSB 6251 Concentrated 1:250-1:1000 0.5 ml
BSB 6252 Concentrated 1:250-1:1000 1.0 ml
BSB 6253 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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