IHC of MCM2 on an FFPE Anal Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation MCM-2 (mini-chromosome maintenance 2) is a human gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex, and may be involved in the formation of replication forks and in the recruitment of other DNA replication-related proteins. This protein forms a complex with MCM-4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by protein kinases CDC2 and CDC7.MCM-2 is essential for eukaryotic DNA replication and drives the formation of pre-replicative complexes, which is the key first step during the G1 phase. Therefore, altered MCM-2 expression may be a hallmark of cell-cycle deregulation, which could be the most essential mechanism in the development and progression of human cancers. MCM2 has been identified by DNA microarray and transcriptional profiling as a gene that is over-expressed in Cervical Carcinomas. This protein is over-expressed in Cervical Dysplasia as a result of HPV infection. The over-expression of MCM-2 provides the link between oncogenic HPV infection and the molecular event of Cervical Dysplasia.
Antibody Type Rabbit Monoclonal Clone RBT-MCM2
Isotype IgG Reactivity Paraffin, Frozen
Localization Nuclear Control HSIL, Cervical and Breast Cancer
Presentation MCM2 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6331 Prediluted Ready-To-Use 3.0 ml
BSB 6332 Prediluted Ready-To-Use 7.0 ml
BSB 6333 Prediluted Ready-To-Use 15.0 ml
BSB 6334 Concentrated 1:100-1:500 0.1 ml
BSB 6335 Concentrated 1:100-1:500 0.5 ml
BSB 6336 Concentrated 1:100-1:500 1.0 ml
BSB 6337 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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