IHC of Kappa on a FFPE Kidney Tissue

IF of Kappa on a Frozen Colon Tissue (IF with FluoroMounter DAPI)

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use.

This antibody is intended for use in Immunohistochemical (IHC) and Immunofluorescence (IF) applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.

Summary and Explanation Kappa detects surface immunoglobulin on normal and neoplastic B-cells. In paraffin-embedded tissue, Kappa exhibits strong staining of kappa-positive plasma cells and cells that have absorbed exogenous immunoglobulin.

 

When studying B-cell neoplasms, the determination of light-chain ratios remains the centerpiece. This is sound reasoning because most B-cell Lymphomas express either Kappa or Lambda light chains, whereas reactive proliferations display a mixture of Kappa and Lambda-positive cells. If only a single light-chain type is detected, a lympho-proliferative disorder is very likely. Monoclonality is determined by a Kappa-Lambda ratio greater than or equal to 3:1, a Lambda-Kappa ratio greater than or equal to 2:1, or a monoclonal population of 75% or more of the total population.

In IgG-dominant immune complex-mediated glomerulonephritis, there are multiple pathological findings that strongly suggest the diagnosis of Lupus Nephritis including immunofluorescence staining for IgG, IgM, IgA, Kappa or Lambda, C3 and C1.

Antibody Type Rabbit Polyclonal Clone Polyclonal
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Tonsil, Lymph Node
Presentation Kappa is a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3086 Prediluted Ready-To-Use* 3.0 ml
BSB 3087 Prediluted Ready-To-Use* 7.0 ml
BSB 3088 Prediluted Ready-To-Use* 15.0 ml
BSB 3089 Concentrated 1:500 / 1:5 0.1 ml
BSB 3090 Concentrated 1:500 / 1:5 0.5 ml
BSB 3091 Concentrated 1:500 / 1:5 1.0 ml
BSB 3688 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

*Ready-to-Use, for IHC only!