IHC of IgM on a FFPE Kidney Tissue

IF of IgM on a Frozen Lupus Erythematosus Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use.

This antibody is intended for use in Immunohistochemical (IHC) and Immunofluorescence (IF) applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.

Summary and Explanation IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, normally as a pentamer or occasionally as a hexamer. It has a large molecular mass of approximately 900 kDa (in its pentamer form). In germline cells, the gene segment encoding the constant region of the heavy chain is positioned first among other constant region gene segments. For this reason, IgM is the first immunoglobulin expressed by mature B-cells.

IgM antibody reacts with surface immunoglobulin IgM mu chains. IgM is one of the predominant surface immunoglobulins on B-lymphocytes, and is useful when identifying Leukemias, Plasmacytomas, and B-cell lineage derived Hodgkin’s Lymphomas. Due to the restricted expression of heavy and light chains in these diseases, demonstration of B-cell Lymphomas is possible with clonal gene-rearrangement studies. Lupus nephritis is an inflammation of the kidneys caused by Systemic Lupus Erythematosus. Immunofluorescence reveals positively for IgG, IgA, IgM, C3, and C1q. Clinically, hematuria and proteinuria are present, with or without nephrotic syndromes. Immunoglobulin M (IgM) nephropathy is an uncommon glomerular disease characterized by IgM deposits in the mesangium.

Antibody Type Rabbit Polyclonal Clone Polyclonal
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Tonsil, Lymph Node, Spleen, Kidney, Colon
Presentation IgM is a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3080 Prediluted Ready-To-Use* 3.0 ml
BSB 3081 Prediluted Ready-To-Use* 7.0 ml
BSB 3082 Prediluted Ready-To-Use* 15.0 ml
BSB 3083 Concentrated 1:100 / 1:40 0.1 ml
BSB 3084 Concentrated 1:100 / 1:40 0.5 ml
BSB 3085 Concentrated 1:100 / 1:40 1.0 ml
BSB 3086 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

*Ready-to-Use, for IHC only!