IHC of IgG on a FFPE Tonsil Tissue

IF of IgG on a Frozen Lupus Erythematosus Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use.

This antibody is intended for use in Immunohistochemical (IHC) and Immunofluorescence (IF) applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.

Summary and Explanation IgG is a monomeric immunoglobulin, comprised of two heavy chains and two light chains. This is the most abundant immunoglobulin and is approximately equally distributed in blood and tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the placenta and bind to many kinds of pathogens. IgG protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralization of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

IgG antibody reacts with surface immunoglobulin IgG gamma chains. This antibody is useful when identifying Leukemias, Plasmacytomas, and B-cell lineage derived Hodgkin’s Lymphomas. Due to the restricted expression of heavy and light chains in these diseases, demonstration of B-cell Lymphomas is possible with clonal gene-rearrangement studies. Lupus nephritis is an inflammation of the kidneys caused by Systemic Lupus Erythematosus. Immunofluorescence reveals positively for IgG, IgA, IgM, C3, and C1q. Clinically, hematuria and proteinuria are present, with or without nephrotic syndromes. Mesangial IgG glomerulonephritis has been recently recognized as a distinct type of glomerulonephritis. The morphologic criteria detected in these patients included mesangial dense deposits by ultra­structural studies, which were predominantly positive for IgG by immunofluorescence.

Antibody Type Rabbit Polyclonal Clone Polyclonal
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Tonsil, Lymph Node, Kidney, Spleen
Presentation IgG is a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3074 Prediluted Ready-To-Use* 3.0 ml
BSB 3075 Prediluted Ready-To-Use* 7.0 ml
BSB 3076 Prediluted Ready-To-Use* 15.0 ml
BSB 3077 Concentrated 1:500 / 1:10 0.1 ml
BSB 3078 Concentrated 1:500 / 1:10 0.5 ml
BSB 3079 Concentrated 1:500 / 1:10 1.0 ml
BSB 3086 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

*Ready-to-Use, for IHC only!