IHC of HER-2 neu Phospho RMab on an FFPE Breast Carcinoma Tissue
|Intended Use||For Analyte Specific Reagent|
|Summary and Explanation||HER2 / ErbB2 is one of four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases .The kinase activity of ErbB2 can be activated without a ligand if it is overexpressed, and by association with other ErbB proteins. Autophosphorylation did not modulate receptor turnover. A Tyr–Phe substitution of ErbB2 Tyr-877 homologous to pp60c-src Tyr-416 did not alter ErbB2 biological and biochemical properties, thus excluding the possibility that phosphorylation of this residue, located in the kinase domain, modulates ErbB2 gp185 catalytic function.
HER2 is overexpressed in 25–30% of all breast cancers, including primary as well as metastatic breast tumors. HER2 has been widely investigated as a prognostic indicator. Detection of phosphorylated HER2 may aid in predicting breast cancer progression.
|Antibody Type||Rabbit Monoclonal||Clone||EP123|
|Presentation||HER-2 neu phospho is a rabbit monoclonal antibody derived from cell culture
supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer
pH 7.5, containing BSA and sodium azide as a preservative.
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|