IHC of GATA3 on a FFPE Breast Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

Trans-acting T-cell-specific transcription factor, GATA-3 is, a protein that in humans is encoded by the GATA3 gene. GATA-3 b regulates luminal epithelial cell differentiation in the mammary gland, is an important regulator of T cell development and plays an important role in endothelial cell biology.

GATA-3 is one of the three genes mutated in >10% of breast cancers. Nuclear expression of GATA-3 in breast cancer is considered a marker of luminal cancer in ER+ cancer and luminal androgen responsive cancer in ER-/AR+ tumors. It is highly coexpressed with FOXA1 and serves as negative predictor of basal subtype and HER-2 and is also considered a strong predictor of taxane and platin salts insensitivity.

GATA3 expression is found in urothelial carcinoma, especially in invasive and high grade tumors. Therefore, anti-GATA3 can be used in a panel of antibodies for diagnosis of unknown primary carcinoma, when carcinomas of the breast or bladder are a possibility. Studies have also shown the utility of GATA-3 in differntiating urothelial carcinoma from prostate adenocarcinoma and squamous cell carcinomas of the uterine, cervix, anus and lung.

Antibody Type Rabbit Monoclonal Clone EP368
Isotype IgG Reactivity Paraffin, Frozen
Localization Nuclear Control Breast, Skin, Cervix, Breast Carcinoma
Presentation GATA3 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3328 Prediluted Ready-To-Use 3.0 ml
BSB 3329 Prediluted Ready-To-Use 7.0 ml
BSB 3330 Prediluted Ready-To-Use 15.0 ml
BSB 3331 Concentrated 1:50-1:200 0.1 ml
BSB 3332 Concentrated 1:50-1:200 0.5 ml
BSB 3333 Concentrated 1:50-1:200 1.0 ml
BSB 3334 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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