IHC of Fli-1 on an FFPE Angiosarcoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Fli-1 protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Approximately 90% of Ewing’s Sarcoma/Primitive Neuroectodermal Tumors (ES/PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein, which can be detected by this antibody. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. Fli-1 has been found to be expressed in the great majority of vascular tumors including Angiosarcomas, Hemangioendotheliomas, Hemangiomas, and Kaposi’s Sarcomas. It has been reported that the high sensitivity and specificity of Fli-1 is equal to or exceeds that of the established vascular markers, CD31, CD34, and Factor VIII. As the first nuclear marker of endothelium (rather than cytoplasmic or membranous), Fli-1 immunostaining also generally lacks cytoplasmic staining artifacts that are the result of endogenous peroxidases or biotin.
Antibody Type Mouse Monoclonal Clone G146-222
Isotype IgG2b Reactivity Paraffin, Frozen
Localization Nuclear Control Adrenal Gland, Fallopian Tube, Placenta, Cervix, Angiosarcoma, PNET, Hemangiomas
Presentation Fli-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 5519 Prediluted Ready-To-Use 3.0 ml
BSB 5520 Prediluted Ready-To-Use 7.0 ml
BSB 5521 Prediluted Ready-To-Use 15.0 ml
BSB 5522 Concentrated 1:25-1:100 0.1 ml
BSB 5523 Concentrated 1:25-1:100 0.5 ml
BSB 5524 Concentrated 1:25-1:100 1.0 ml
BSB 5525 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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