IHC of Fascin on an FFPE Hodgkin’s Lymphoma Tissue

IHC of Fascin on an FFPE Hodgkin’s Lymphoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s Lymphoma are positive for Fascin staining. Epstein-Barr virus may induce expression of Fascin in B-cells. Fascin is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin’s Disease. This marker might be helpful in distinguishing between Hodgkin’s Disease and Non-Hodgkin Lymphoma in difficult cases. Also, the lack of expression of Fascin in the neoplastic follicles in Follicular Lymphoma can be helpful in distinguishing these lymphomas from reactive Follicular Hyperplasia in which the number of follicular dendritic cells is normal or increased.
Antibody Type Mouse Monoclonal Clone BSB-36
Isotype IgG2a/K Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Hodgkin’s Lymphoma, Lymph Node, Tonsil
Presentation
Presentation Fascin is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 5512 Prediluted Ready-To-Use 3.0 ml
BSB 5513 Prediluted Ready-To-Use 7.0 ml
BSB 5514 Prediluted Ready-To-Use 15.0 ml
BSB 5515 Concentrated 1:250-1:1000 0.1 ml
BSB 5516 Concentrated 1:250-1:1000 0.5 ml
BSB 5517 Concentrated 1:250-1:1000 1.0 ml
BSB 5518 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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