IHC of EMA on an FFPE Ovarian Adenocarcinoma Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationEpithelial Membrane Antigen (EMA) antibody is a mucin-like glycoprotein, shown to be useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and squamous epithelium. Hepatocellular Carcinoma, Adrenal Carcinoma and Embryonal Carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms. The absence of EMA can also be of value since negative EMA is characteristic of some tumors including Adrenal Carcinoma, Seminomas, Paraganglioma and Hepatoma.
Antibody TypeMouse MonoclonalCloneE29
IsotypeIgG2a/KReactivityParaffin, Frozen
LocalizationCytoplasmic, MembranousControlBreast, Skin, Colon, Kidney, Cervix
PresentationEMA is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 5477PredilutedReady-To-Use3.0 ml
BSB 5478PredilutedReady-To-Use7.0 ml
BSB 5479PredilutedReady-To-Use15.0 ml
BSB 5480Concentrated1:500-1:20000.1 ml
BSB 5481Concentrated1:250-1:10000.5 ml
BSB 5482Concentrated1:250-1:10001.0 ml
BSB 5483Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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