IHC of DOG-1 on an FFPE GIST Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

DOG1 (discovered on GIST 1), a cell-surface protein of unknown function, is expressed strongly on the cell surface of GISTs and is rarely expressed in other soft tissue tumors. Among GIST cases with c-Kit mutations, the DOG1 antibody identified 11% more cases than a c-Kit antibody.

DOG1 identifies the vast majority of both c-Kit negative and PDGFRA mutated GIST cases that may still benefit from imatinib mesylate (Gleevac), an inhibitor of the kit tyrosine kinase. In addition, DOG1 immunoreactivity is seen in fewer cases of mesenchymal and epithelial tumors, and melanomas when compared with c-Kit. The use of this highly-sensitive and specific novel marker should increase the accuracy of GIST diagnosis.

Antibody Type Rabbit Monoclonal Clone EP332
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Salivary Gland, Breast, GIST
Presentation DOG-1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2901 Prediluted Ready-To-Use 3.0 ml
BSB 2902 Prediluted Ready-To-Use 7.0 ml
BSB 2903 Prediluted Ready-To-Use 15.0 ml
BSB 2904 Concentrated 1:25-1:100 0.1 ml
BSB 2905 Concentrated 1:25-1:100 0.5 ml
BSB 2906 Concentrated 1:25-1:100 1.0 ml
BSB 2907 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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