IHC of Cytokeratin 8 & 18 on an FFPE Colon Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Cytokeratin 8 belongs to the Type II (basic) subfamily of high molecular-weight keratins and exists in combination with Cytokeratin 18 (Type I [acidic] subfamily of low molecular weight keratins). They are perhaps the most commonly found products of the intermediate filament gene family, and are expressed in single-layer epithelial tissues of the body. Cytokeratins 8 and 18 can be found in most simple epithelium (e.g., thyroid, female breast, gastrointestinal tract, and respiratory tract). Adenocarcinomas and most Non-keratinizing Squamous Carcinomas will stain, but Keratinizing Squamous Carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells will stain. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.
Antibody Type Mouse Monoclonal Clone B22.1/B23.1
Isotype IgG1 Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Breast, Ovary, GI, Prostate, Pancreas, Salivary Gland
Presentation Cytokeratin 8 & 18 is a cocktail of mouse monoclonal antibodies derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 5414 Prediluted Ready-To-Use 3.0 ml
BSB 5415 Prediluted Ready-To-Use 7.0 ml
BSB 5416 Prediluted Ready-To-Use 15.0 ml
BSB 5417 Concentrated 1:250-1:1000 0.1 ml
BSB 5418 Concentrated 1:250-1:1000 0.5 ml
BSB 5419 Concentrated 1:250-1:1000 1.0 ml
BSB 5420 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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