IHC of Cytokeratin 8 & 18 on an FFPE Colon Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Cytokeratin 8 belongs to the Type II (basic) subfamily of high molecular-weight keratins and exists in combination with Cytokeratin 18 (Type I [acidic] subfamily of low molecular weight keratins). They are perhaps the most commonly found products of the intermediate filament gene family, and are expressed in single-layer epithelial tissues of the body.
Cytokeratins 8 and 18 can be found in most simple epithelium (e.g., thyroid, female breast, gastrointestinal tract, and respiratory tract). Adenocarcinomas and most Non-keratinizing Squamous Carcinomas will stain, but Keratinizing Squamous Carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells will stain. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.
Antibody Type Rabbit Monoclonal Clone EP17 & EP30
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic Control Breast, Ovary, GI, Prostate, Pancreas, Salivary Gland, Colon
Presentation CK8/18 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2049 Prediluted Ready-To-Use 3.0 ml
BSB 2050 Prediluted Ready-To-Use 7.0 ml
BSB 2051 Prediluted Ready-To-Use 15.0 ml
BSB 2052 Concentrated 1:25-1:100 0.1 ml
BSB 2053 Concentrated 1:25-1:100 0.5 ml
BSB 2054 Concentrated 1:25-1:100 1.0 ml
BSB 2055 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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