IHC of Cytokeratin 7 & CDX2 on an FFPE Colon Carcinoma Metastasis to Lung Tissue

IHC of Cytokeratin 7 & CDX2 on an FFPE Colon Carcinoma Metastasis to Lung Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Cytokeratin 7 (CK7) reacts with proteins that are found in most ductal, glandular and transitional epithelium of the urinary tract and bile duct epithelial cells. CK7 distinguishes between lung and breast epithelium that stain positive, and colon and prostate epithelial cells that are negative. CDX2 is a caudal-type homeobox gene that encodes an intestine-specific transcription factor expressed early in intestinal development and that may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells.
The Cytokeratin 7 antibody has been shown to label the rete epithelium in the testis, epididymis epithelium, and the surface epithelium of the stomach and duodenum. Transitional-cell Carcinomas are positive and Prostate Cancers are negative. Anti-CDX2 antibody has been useful in distinguishing the gastrointestinal origin of Metastatic Adenocarcinomas and Carcinoids. A high percentage of Mucinous Carcinomas of the Ovary also stain positively with this antibody, as well as Carcinomas from the upper gastrointestinal tract.
Antibody Type Rabbit Monoclonal Clone EP16 & EP25
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Nuclear Control Colon, Breast, Ovary, Lung Carcinoma
Presentation CK7 & CDX2 is a rabbit monoclonal cocktail antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6653 Prediluted Ready-To-Use 3.0 ml
BSB 6654 Prediluted Ready-To-Use 7.0 ml
BSB 6655 Prediluted Ready-To-Use 15.0 ml
BSB 6656 Concentrated 1:25-1:100 0.1 ml
BSB 6657 Concentrated 1:25-1:100 0.5 ml
BSB 6658 Concentrated 1:25-1:100 1.0 ml
BSB 6659 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Related Products