IHC of CD45 RO on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation The CD45 family consists of multiple members that are all products of a single complex gene. Three isoforms of CD45 exist: on B-lymphocytes, where the protein is called B220 (its molecular mass is 220 kDA); on naive T-lymphocytes, where it is called CD45 RA, and on activated and memory T-lymphocytes, where it is called CD45 RO. CD45RO is a single-chain, transmembraneous glycoprotein which represents the low molecular weight isoform of the Leukocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T-cells, virtually all T-cells in skin reactive infiltrates, and the majority of T-cell malignancies. It is also found on a subset of B-cells and on exceptional B-cell Lymphomas. CD45RO (T-Cell, Pan) antibody reacts with thymocytes and activated T-cells, but only on a subpopulation of resting T-cells. This antibody shows no reactivity with B-cells, making it a good marker for T-cell tumors to be phenotyped. In addition, granulocytes and monocytes are also labeled with this antibody. T-Cell, Pan has been designated as CD45RO at The International Leukocyte Typing Workshop.
Antibody Type Mouse Monoclonal Clone UCHL-1
Isotype IgG2a/K Reactivity Paraffin, Frozen
Localization Membranous Control Tonsil, Lymph Node
Presentation CD45RO is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 5260 Prediluted Ready-To-Use 3.0 ml
BSB 5261 Prediluted Ready-To-Use 7.0 ml
BSB 5262 Prediluted Ready-To-Use 15.0 ml
BSB 5263 Concentrated 1:250-1:1000 0.1 ml
BSB 5264 Concentrated 1:250-1:1000 0.5 ml
BSB 5265 Concentrated 1:250-1:1000 1.0 ml
BSB 5266 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Related Products