IHC of CD33 on a FFPE Colon Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||
CD33 (gp62 or siglec-3) is a glycosylated transmembrane protein that is a member of the sialic acid-binding immunoglobulin-like lectin (siglec) family. The genomic locus of this protein has been mapped to chromosome 19q13.1-3.5. The function of CD33 is not known, but it may have a role in cell-to-cell adhesion. In maturing granulocytic cells, there is progressive down-regulation of CD33 from the blast stage to mature neutrophils. However, in monocytes and macrophages/histiocytes, strong expression of CD33 is maintained throughout maturation.
Detection of CD33 using monoclonal antibodies has been a critical component in immunophenotyping acute leukemias, particularly Acute Myeloid Leukemias.
|Antibody Type||Rabbit Monoclonal||Clone||RBT-CD33|
|Localization||Membranous||Control||Placenta, Myometrium, Lung, Colon, Spleen, Lymph Node, Tonsil, Acute Myeloid Leukemia|
|Presentation||CD33 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.|
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|