IHC of CD25 on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation CD25 is the alpha chain of the IL-2 receptor. It is a Type I transmembrane protein present on activated T-cells, activated B-cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. It is expressed in most B-cell neoplasms, some Acute Non-lymphocytic Leukemias, and Neuroblastomas. Expression of CD25 is a reliable diagnostic tool for distinguishing neoplastic mast-cell aggregates from reactive proliferations, and has, therefore, recently become a minor criterion for the diagnosis of Systemic Mastocytosis (SM). Anti-CD25 antibodies have also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis. Quantitation of regulatory T-cells (Treg) in the setting of hepatocellular carcinoma has been used as an independent predictive factor for tumor recurrence after hepatic resection for HCC. Also, the percentage of tumor-infiltrating CD25+FOXP3+ regulatory T-cells among tumor cells, inside tumor parenchyma and at its periphery are significantly higher in recurrent Cutaneous Melanoma than in Non-recurrent Melanoma.
Antibody Type Mouse Monoclonal Clone 4C9
Isotype IgG2b Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Tonsil, Small Bowel, Spleen, Mastocytosis, Hodgkin’s Lymphoma
Presentation CD25 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6317 Prediluted Ready-To-Use 3.0 ml
BSB 6318 Prediluted Ready-To-Use 7.0 ml
BSB 6319 Prediluted Ready-To-Use 15.0 ml
BSB 6320 Concentrated 1:10-1:50 0.1 ml
BSB 6321 Concentrated 1:10-1:50 0.5 ml
BSB 6322 Concentrated 1:10-1:50 1.0 ml
BSB 6323 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
Related Products