IHC of CD16 on a FFPE Bone Marrow Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

CD16 is a low affinity Fc receptor, found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies which then activates NK cells for antibody-dependent cell-mediated cytotoxicity. A lack of CD16 in a given population of neutrophils may indicate prematurity, as could be caused by a left shift due to neutrophilic leukocytosis induced by tissue necrosis or bacterial infection.

The IHC of CD16 is useful in the differential diagnosis of hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from other peripheral T-cell lymphomas, such as mucosal and cutaneous gamma delta T-cell lymphoma. A significant decrease can be seen in the number of granulocytes expressing CD16 in chronic myelomonocytic leukemia compared to chronic myelogenous leukemia and control bone marrow biopsy, probably related to dysgranulopoiesis. It has also been demonstrated that colorectal carcinoma patients with high CD16+ cell infiltration is associated with improved overall survival after adjusting for known prognostic factors and this association was independent from CD8+ lymphocyte infiltration and presence of metastases.

Antibody Type Rabbit Monoclonal Clone EP364
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Placenta, Liver, Breast, Spleen, Thymus, Lung
Presentation CD16 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 3321 Prediluted Ready-To-Use 3.0 ml
BSB 3322 Prediluted Ready-To-Use 7.0 ml
BSB 3323 Prediluted Ready-To-Use 15.0 ml
BSB 3324 Concentrated 1:50-1:200 0.1 ml
BSB 3325 Concentrated 1:50-1:200 0.5 ml
BSB 3326 Concentrated 1:50-1:200 1.0 ml
BSB 3327 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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