IHC of CD103 on an FFPE Colon Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation

Integrin, alpha E (ITGAE), also known as CD103, is an integrin protein that in human is encoded by the ITGAE gene. CD103 binds integrin beta 7 (β7– ITGB7) to form the complete heterodimeric integrin molecule αEβ7. CD103 is expressed widely on intraepithelial lymphocyte (IEL) T cells (both α β T cells and γ δ T cells) and on some peripheral regulatory T cells (Tregs). It has also been reported on lamina propria T cells. A subset of dendritic cells in the gut mucosa and in mesenteric lymph nodes also expresses this marker and is known as CD103 DCs. The chief ligand for αEβ7 is E-cadherin, an adhesion molecule found on epithelial cells.

CD103 has been found in mononuclear cells in the interfollicular area of lymph nodes and in intraepithelial cells in the overlying mucosa located primarily toward the basal layer of the tonsil. CD103 is useful in identifying Hairy Cell Leukemia, which is positive for this marker in most cases in contrast to other hematologic malignancies which are negative for CD103, with the exception of Splenic Marginal Zone Lymphoma, which rarely expresses CD103.  The high sensitivity of anti-CD103 for Hairy Cell Leukemia makes this marker valuable when distinguishing this malignancy from other B-cell neoplasms.

Antibody Type Rabbit Monoclonal Clone EP206
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Skin, Colon, Tonsil, Thymus, Spleen, Hairy Cell Leukemia
Presentation CD103 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2859 Prediluted Ready-To-Use 3.0 ml
BSB 2860 Prediluted Ready-To-Use 7.0 ml
BSB 2861 Prediluted Ready-To-Use 15.0 ml
BSB 2862 Concentrated 1:25-1:100 0.1 ml
BSB 2863 Concentrated 1:25-1:100 0.5 ml
BSB 2864 Concentrated 1:25-1:100 1.0 ml
BSB 2865 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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