IHC of CA15-3 on an FFPE Breast Tissue

United States
Research Use
Safety Data Sheet


Intended Use For In Vitro Diagnostic Use
Summary and Explanation This antibody has been used for evaluating the primary site of a metastatic carcinoma of unknown origin and distinguishing between benign and malignant lesions. It is believed that CA15-3 reacts primarily with the DF3-antigen, a 300 kDa mucin-like glycoprotein present on the apical border of secretory mammary epithelial cells. CA15-3 has been detected with immunohistochemistry in a wide spectrum of carcinomas, including Breast Carcinomas (ductal and lobular), Sarcomas (Synovial Sarcoma and Malignant Fibrous Histiocytomas), and Lung Carcinomas. CA15-3 can be used as a supplementary marker for epithelial differentiation. CA15-3 does not stain Melanomas or Ewing’s Sarcomas. Approximately 30% of Hepatocellular Carcinomas are positive for CA15-3.
Antibody Type Mouse Monoclonal Clone DF3
Isotype IgG1 Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Kidney, Breast, Pancreas, Lymph Node, Cervix, Salivary Gland, Bladder TCC
Presentation CA15-3 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Catalog No. Antibody Type Dilution Volume/QTY
BSB 5099 Prediluted Ready-To-Use 3.0 ml
BSB 5100 Prediluted Ready-To-Use 7.0 ml
BSB 5101 Prediluted Ready-To-Use 15.0 ml
BSB 5102 Concentrated 1:250-1:1000 0.1 ml
BSB 5103 Concentrated 1:250-1:1000 0.5 ml
BSB 5104 Concentrated 1:250-1:1000 1.0 ml
BSB 5105 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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