IHC of C4d on a FFPE Spleen Tissue

IF of C4d on a Frozen Spleen Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use.

This antibody is intended for use in Immunohistochemical (IHC) and Immunofluorescence (IF) applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.

Summary and Explanation Complement component 4, or C4, plays a central role in the complement system. C4d is the final proteolytic remnant of deposited C4b on endothelium and remains covalently attached to endothelium for little more than a week. It is easily detectable by Immunohistochemistry.

Anti-C4d combined with anti-C3d can be utilized as a tool for diagnosis of AR (Acute Rejection) and warrant prompt and aggressive anti-rejection treatment. C4d can be detected in peritubular capillaries in both chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival and is an aid in treating acute rejection.

Antibody Type Rabbit Monoclonal Clone EP272
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Tonsil, Lymph Node, Spleen, Kidney, Kidney Transplant Rejection
Presentation C4d is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2831 Prediluted Ready-To-Use* 3.0 ml
BSB 2832 Prediluted Ready-To-Use* 7.0 ml
BSB 2833 Prediluted Ready-To-Use* 15.0 ml
BSB 2834 Concentrated 1:100 / 1:50 0.1 ml
BSB 2835 Concentrated 1:100 / 1:50 0.5 ml
BSB 2836 Concentrated 1:100 / 1:50 1.0 ml
BSB 2837 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.

*Ready-to-Use, for IHC only!