BOB.1

IHC of BOB.1 on an FFPE Tonsil Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation The BOB.1 protein is a co-activator that interacts with OCT-1 and/or OCT-2 transcription factors, and is critical in germinal center formation and immunoglobulin production. The strongest expression of BOB.1 is found in the germinal center, mantle-zone B cells, and plasma cells. Because BOB.1/OBF.1 are germinal center derived, L&H cells in Nodular Lymphocyte Predominant Hodgkin’s Lymphoma are consistently immunoreactive for BOB.1. Conversely, the Hodgkin/Reed-Sternberg cells in classical Hodgkin’s Lymphoma either do not express both or express only one of the two proteins or neither.
In Diffuse Large B-cell Lymphomas, the highest expression levels for BOB.1/OBF.1 are reported in Follicular Center Lymphomas, Diffuse Large B-cell Lymphomas, and Burkitt Lymphomas. B-CLL, MALT-type, and Mantle Cell Lymphomas score negative or display a heterogenous/weaker activity. The strong nuclear expression of BOB.1 and Oct-2 by Germinal Center Derived Lymphomas makes these antibodies a novel class of broad spectrum B-lineage immunohistochemical markers in the differential diagnosis of Lymphomas, specifically between Primary Mediastinal B-cell Lymphoma from classical Hodgkin’s Disease .
Antibody Type Rabbit Monoclonal Clone EP114
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Nuclear Control Tonsil, Lymph Node
Presentation BOB.1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6380 Prediluted Ready-To-Use 3.0 ml
BSB 6381 Prediluted Ready-To-Use 7.0 ml
BSB 6382 Prediluted Ready-To-Use 15.0 ml
BSB 6383 Concentrated 1:100-1:500 0.1 ml
BSB 6384 Concentrated 1:100-1:500 0.5 ml
BSB 6385 Concentrated 1:100-1:500 1.0 ml
BSB 6386 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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