BAX_RMab

IHC of Bax on an FFPE Hodgkin’s Lymphoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Bax is a protein of the bcl-2 gene family. It promotes apoptosis by competing with bcl-2 proper. The Bax gene contains a small promoter element that complements a binding domain on the multi-faceted p53 tumor suppressor. Wild-type p53 has been demonstrated to upregulate the transcription of a chimeric reporter plasmid, utilizing the consensus promoter sequence of Bax approx. 50-fold over mutant p53. Mutations in this consensus sequence eliminate transcription of the reporter gene. Thus, it is likely that p53 promotes Bax’s apoptotic faculties in vivo as a primary transcription factor. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial mem-branes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacement of Bax. The displacement of Bax allows apoptosis to proceed.
Antibody Type Rabbit Monoclonal Clone E63
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Membranous Control Breast, Tonsil, Cervix, Hodgkin’s Lymphoma
Presentation Bax is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 6078 Prediluted Ready-To-Use 3.0 ml
BSB 6079 Prediluted Ready-To-Use 7.0 ml
BSB 6080 Prediluted Ready-To-Use 15.0 ml
BSB 6081 Concentrated 1:50-1:200 0.1 ml
BSB 6082 Concentrated 1:50-1:200 0.5 ml
BSB 6083 Concentrated 1:50-1:200 1.0 ml
BSB 6084 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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