IHC of BAP1 on a FFPE Mesothelioma Tissue

Specification Sheets
Safety Data Sheet
Intended UseFor In Vitro Diagnostic Use
Summary and ExplanationBAP1 or BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) is a deubiquitinating enzyme that in humans is encoded by the BAP1 gene. Recent studies have shown that BAP1 and its fruit fly homolog, Calypso, are members of the polycomb-group proteins (PcG) of highly conserved transcriptional repressors required for long-term silencing of genes that regulate cell fate determination, stem cell pluripotency, and other developmental processes.

In cancer, BAP1 can function both as a Tumor suppressor and as a metastasis suppressor. Exome sequencing identified inactivating mutations in BAP1 in 47% of Uveal melanomas, and BAP1 mutation have been found to be strongly associated with metastasis. The atypical melanocytic lesions resemble Spitz nevi and have been characterized as “atypical Spitz tumors” (ASTs), although they have a unique histology and exhibit both BRAF and BAP1 mutations.

BAP1 mutations have been identified in aggressive Mesotheliomas with similar mutations as seen in melanomas. Sequencing studies have been used to identify germline mutations in BAP1 in families with genetic predispositions to mesothelioma and melanocytic skin tumors. Mutations in the tumor suppressor gene BAP1 occur in approximately 15% of clear cell renal cell carcinoma cases. Sequencing efforts demonstrated worse outcomes in patients with BAP1 mutated clear cell renal cell carcinoma. Immunohistochemistry for BAP1 is a prognostic biomarker to predict poor oncologic outcomes and adverse clinicopathological features in patients with non-metastatic clear cell renal cell carcinoma. BAP1 assessment using immunohistochemistry on needle biopsy may benefit preoperative risk stratification and guide treatment planning.

Antibody TypeMouse MonoclonalCloneBSB-109
IsotypeIgG1ReactivityParaffin, Frozen
LocalizationNuclearControlTestis, TCC, Mesothelioma
PresentationBAP1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Availability
Catalog No.Antibody TypeDilutionVolume/QTY
BSB 3300PredilutedReady-To-Use3.0 ml
BSB 3301PredilutedReady-To-Use7.0 ml
BSB 3302PredilutedReady-To-Use15.0 ml
BSB 3303Concentrated1:50-1:2000.1 ml
BSB 3304Concentrated1:50-1:2000.5 ml
BSB 3305Concentrated1:50-1:2001.0 ml
BSB 3306Control Slides5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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