Arginase-1

IHC of Arginase-1 on a FFPE Hepatocellular Carcinoma Tissue

Specification Sheets
Safety Data Sheet

SDS

Intended Use For In Vitro Diagnostic Use
Summary and Explanation Arginase-1 is the catalyst for the fifth and final step in the urea cycle, which is a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Arginase-1 works to convert L-arginine into L-ornithine and urea. Arginise-1 is located primarily in the cytoplasm of the liver. Arginase-1 consists of three tetramers, and the enzyme requires a two-molecule metal clusterof manganese in order to maintain proper function. These Mn2+ ions coordinate with water, orienting and stabilizing the molecule and allowing water to act as anucelophile and attack L-arginine, hydrolyzing it into ornithine and urea.Arginase-1 is abundantly expressed in the liver and it represents a sensitive and specific marker of benign and malignant hepatocytes. In sections of normal liver, anti-arginase 1 produces strong, diffuse cytoplasmic reactivity in all hepatocytes throughout the lobule. In a small percentage of cases, patchy nuclear reactivityis also evident in hepatocytes along with the strong cytoplasmic reactivity. Hepatocellular carcinoma usually shows higher protein expression of ARG1 than normal liver cells
Antibody Type Rabbit Monoclonal Clone EP 261
Isotype IgG Reactivity Paraffin, Frozen
Localization Cytoplasmic, Nuclear Control Liver, Hepatocellular Carcinoma
Presentation Arginase-1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5,
containing BSA and sodium azide as a preservative.
Availability
Catalog No. Antibody Type Dilution Volume/QTY
BSB 2447 Prediluted Ready-To-Use 3.0 ml
BSB 2448 Prediluted Ready-To-Use 7.0 ml
BSB 2449 Prediluted Ready-To-Use 15.0 ml
BSB 2450 Concentrated 1:50-1:200 0.1 ml
BSB 2451 Concentrated 1:50-1:200 0.5 ml
BSB 2452 Concentrated 1:50-1:200 1.0 ml
BSB 2453 Control Slides 5
Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.
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