IHC of Arginase-1 on a FFPE Hepatocellular Carcinoma Tissue
|Intended Use||For In Vitro Diagnostic Use|
|Summary and Explanation||Arginase-1 is the catalyst for the fifth and final step in the urea cycle, which is a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Arginase-1 works to convert L-arginine into L-ornithine and urea. Arginise-1 is located primarily in the cytoplasm of the liver. Arginase-1 consists of three tetramers, and the enzyme requires a two-molecule metal clusterof manganese in order to maintain proper function. These Mn2+ ions coordinate with water, orienting and stabilizing the molecule and allowing water to act as anucelophile and attack L-arginine, hydrolyzing it into ornithine and urea.Arginase-1 is abundantly expressed in the liver and it represents a sensitive and specific marker of benign and malignant hepatocytes. In sections of normal liver, anti-arginase 1 produces strong, diffuse cytoplasmic reactivity in all hepatocytes throughout the lobule. In a small percentage of cases, patchy nuclear reactivityis also evident in hepatocytes along with the strong cytoplasmic reactivity. Hepatocellular carcinoma usually shows higher protein expression of ARG1 than normal liver cells|
|Antibody Type||Rabbit Monoclonal||Clone||EP 261|
|Localization||Cytoplasmic, Nuclear||Control||Liver, Hepatocellular Carcinoma|
|Presentation||Arginase-1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5,
containing BSA and sodium azide as a preservative.
|Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.|